Anti-BIG-2 antibody [EPR8735] (ab137107)
![Anti-BIG-2 antibody [EPR8735] (ab137107)](https://www.abcam.com/ps/products/137/ab137107/Images/ab137107-151713-anti-big-2-antibody-epr8735-western-blot.jpg "Anti-BIG-2 antibody [EPR8735] (ab137107)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8735] to BIG-2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-BIG-2 antibody [EPR8735]
See all BIG-2 primary antibodies -
Description
Rabbit monoclonal [EPR8735] to BIG-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human BIG-2 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- Human fetal brain, Human cerebellum and Mouse brain lysates; Human brain and kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8735 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BIG-2 antibody [EPR8735] (ab137107)All lanes : Anti-BIG-2 antibody [EPR8735] (ab137107) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human cerebellum lysate
Lane 3 : Mouse brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 113 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8735] (ab137107)](https://www.abcam.com/ps/products/137/ab137107/Images/ab137107-151712-anti-big-2-antibody-epr8735-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8735] (ab137107)Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling BIG-2 with ab137107 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8735] (ab137107)](https://www.abcam.com/ps/products/137/ab137107/Images/ab137107-151711-anti-big-2-antibody-epr8735-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8735] (ab137107)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling BIG-2 with ab137107 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-BIG-2 antibody [EPR8735] (ab137107)](https://www.abcam.com/ps/products/137/ab137107/Images/ab137107-4-benefits-of-recombinant-antibodies.png)
Anti-BIG-2 antibody [EPR8735] (ab137107)
