Anti-BIG-2 antibody (ab232782)
Key features and details
- Rabbit polyclonal to BIG-2
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-BIG-2 antibody
See all BIG-2 primary antibodies -
Description
Rabbit polyclonal to BIG-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human BIG-2 aa 700-1000. (Expressed in E.coli).
Database link: Q8IWV2 -
Positive control
- WB: Recombinant human BIG-2 protein; HeLa cell lysate; Pig brain lysate. IHC-P: Human prostate and breast cancer tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab232782 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BIG-2 antibody (ab232782)All lanes : Anti-BIG-2 antibody (ab232782) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Pig brain lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 113 kDa
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Western blot - Anti-BIG-2 antibody (ab232782)Anti-BIG-2 antibody (ab232782) at 2 µg/ml + Recombinant human BIG-2 protein.
Predicted band size: 113 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody (ab232782)Paraffin-embedded human breast cancer tissue stained for BIG-2 using ab232782 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody (ab232782)Paraffin-embedded human prostate tissue stained for BIG-2 using ab232782 at 20 µg/ml in immunohistochemical analysis. DAB staining.
