Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cathepsin D antibody [EPR3054] - BSA and Azide free (ab206472)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3054] to Cathepsin D - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Cathepsin D antibody [EPR3054] - BSA and Azide free
    See all Cathepsin D primary antibodies

  • Description

    Rabbit monoclonal [EPR3054] to Cathepsin D - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human breast ductal infiltrating carcinoma tissue; A431, MCF7 and SKBR3 cell lysates.

  • General notes

    ab206472 is the carrier-free version of ab134169 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab206472 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Cathepsin D antibody [EPR3054] - BSA and Azide free (ab206472)
    Western blot - Anti-Cathepsin D antibody [EPR3054] - BSA and Azide free (ab206472)
    All lanes : Anti-Cathepsin D antibody [EPR3054] - BSA and Azide free (ab206472) at 1/2000 dilution

    Lane 1 : Wild-type A431 whole cell lysate
    Lane 2 : CTSD knockout A431 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 44 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab134169 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab134169 was shown to recognize in wild-type A431 cells as signal was lost at the expected MW in CTSD knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CTSD knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab134169 and ab7291 (Mouse anti-tubulin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134169).

  • Anti-Cathepsin D antibody [EPR3054] - BSA and Azide free (ab206472)
    Anti-Cathepsin D antibody [EPR3054] - BSA and Azide free (ab206472)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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