Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

Order now and get it on Tuesday March 02, 2021

Anti-mtTFA antibody [EPR23548-120] (ab252432)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23548-120] to mtTFA
Suitable for: IP, WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat

Product name

Anti-mtTFA antibody [EPR23548-120]
See all mtTFA primary antibodies
Description

Rabbit monoclonal [EPR23548-120] to mtTFA
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse Rat
IP	Mouse Rat
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: C2C12, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse cerebellum, heart and kidney tissue lysates;, Rat cerebellum and heart tissue lysates. ICC/IF: C2C12 and PC-12 cells. Flow Cyt: C2C12 cells. IP: C2C12 and PC-12 cell lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23548-120
Isotype

IgG
Research areas

Western blot - Anti-mtTFA antibody [EPR23548-120] (ab252432)

All lanes : Anti-mtTFA antibody [EPR23548-120] (ab252432) at 1/1000 dilution

Lane 1 : Mouse cerebellum tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Rat cerebellum tissue lysate
Lane 5 : Rat heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

Predicted band size: 28 kDa
Observed band size: 25 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Flow Cytometry - Anti-mtTFA antibody [EPR23548-120] (ab252432)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labelling mtTFA with ab252432 at 1/600 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR23548-120] (ab252432)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 cells labelling mtTFA with ab252432 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.
Immunoprecipitation - Anti-mtTFA antibody [EPR23548-120] (ab252432)

mtTFA was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast) whole cell lysate using ab252432 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab252432 at 1/500 dilution, followed by VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution.

Lane 1: C2C12 whole cell lysate (Input)
Lane 2: ab252432 IP in C2C12 cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252432 in C2C12 whole cell lysate

Blocking /Diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 32 seconds
Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR23548-120] (ab252432)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 cells labelling mtTFA with ab252432 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.
Immunoprecipitation - Anti-mtTFA antibody [EPR23548-120] (ab252432)

mtTFA was immunoprecipitated from 0.35 mg PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate using ab252432 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab252432 at 1/500 dilution, followed by VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution.

Lane 1: PC-12 whole cell lysate (Input)
Lane 2: ab252432 IP in PC-12 cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252432 in PC-12 whole cell lysate

Blocking /Diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 32 seconds
Western blot - Anti-mtTFA antibody [EPR23548-120] (ab252432)

All lanes : Anti-mtTFA antibody [EPR23548-120] (ab252432) at 1/1000 dilution

Lane 1 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lane 2 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 3 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

Predicted band size: 28 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 25375380)

Exposure times: Lane 1: 3 minutes; Lanes 2-5: 70 seconds.
Anti-mtTFA antibody [EPR23548-120] (ab252432)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com