Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)
![Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)](https://www.abcam.com/ps/products/32/ab32123/Images/ab32123-43955-anti-cd22-phospho-y822-antibody-y506-flow-cytometry.jpg "Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)")
Key features and details
- Rabbit monoclonal [Y506] to CD22 (phospho Y822)
- Suitable for: WB, Flow Cyt, Dot blot
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD22 (phospho Y822) antibody [Y506]
See all CD22 primary antibodies -
Description
Rabbit monoclonal [Y506] to CD22 (phospho Y822) -
Host species
Rabbit -
Specificity
ab32123 recognises CD22 phosphorylation on Tyrosine 822. This antibody is predicted to detect splice isoform 2 based on sequence homology. -
Tested applications
Suitable for: WB, Flow Cyt, Dot blotmore details
Unsuitable for: IHC or IP -
Species reactivity
Reacts with: Human -
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 822 of human CD22
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Positive control
- WB: Ramos and Raji cell lysates treated with Pervanadate. Flow Cyt: K562 cells treated with Pervanadate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
Y506 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)Flow cytometric analysis of permeabilized K562 cells, untreated (green) or Pervanadate treated (red) using ab32123
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![Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)](https://www.abcam.com/ps/products/32/ab32123/Images/ab32123-296745-anti-cd22-phospho-y822-antibody-y506-western-blot.jpg)
Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)All lanes : Anti-CD22 (phospho Y822) antibody [Y506] (ab32123) at 1/1000 dilution
Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate
Lane 3 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)](https://www.abcam.com/ps/products/32/ab32123/Images/ab32123-296730-anti-cd22-phospho-y822-antibody-y506-dot-blot.jpg)
Dot Blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)Dot blot analysis of CD22 (phospho Y822) phospho peptide (Lane 1) and CD22 non-phospho peptide (Lane 2) labelling CD22 (phospho Y822) with ab32123 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)](https://www.abcam.com/ps/products/32/ab32123/Images/ab32123-43956-anti-cd22-phospho-y822-antibody-y506-western-blot.jpg)
Western blot - Anti-CD22 (phospho Y822) antibody [Y506] (ab32123)All lanes : Anti-CD22 (phospho Y822) antibody [Y506] (ab32123) at 1/1000 dilution
Lane 1 : Untreated Raji cell lysate
Lane 2 : Raji cell lysate treated with Pervanadate
Predicted band size: 95 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
