Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755)

Key features and details

Mouse monoclonal [BLCAM/1795] to CD22 - BSA and Azide free
Suitable for: WB, IHC-P, Protein Array
Reacts with: Human
Isotype: IgG1

Product name

Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free
See all CD22 primary antibodies
Description

Mouse monoclonal [BLCAM/1795] to CD22 - BSA and Azide free
Host species

Mouse
Tested applications

Suitable for: WB, IHC-P, Protein Arraymore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment within Human CD22 aa 52-178. The exact sequence is proprietary.
Database link: P20273
Positive control
- Raji and Ramos cell lysates; Human tonsil tissue.
General notes

ab269755 is a carrier free version of ab218340. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.

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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

BLCAM/1795
Isotype

IgG1
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody (ab269755)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human tonsil tissue labeling CD22 with ab218340 at 2 μg/ml dilution, followed by conjugation to the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218340).
Western blot - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755)

All lanes : Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755) at 2 µg/ml

Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate

Predicted band size: 95 kDa
Western blot - Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755)

Anti-CD22 antibody [BLCAM/1795] - BSA and Azide free (ab269755) at 2 µg/ml + Raji (human Burkitt's lymphoma cell line) whole cell lysate

Predicted band size: 95 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody (ab269755)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD22 with ab218340.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218340).
Protein Array - Anti-CD22 antibody (ab269755)

ab218340 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218340).

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