Human ALCAM ELISA Kit (CD166) (ab270205)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.02 ng/ml
- Range: 0.188 ng/ml - 12 ng/ml
- Sample type: Cell culture media, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ALCAM ELISA Kit (CD166)
See all Alcam kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 3 3.2% Inter-assay Sample n Mean SD CV% Serum 8 6.6% -
Sample type
Serum, Cell Lysate, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.02 ng/ml -
Range
0.188 ng/ml - 12 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 119 114% - 123% Cell Lysate 101 95% - 108% Cell culture media 102 101% - 102% Hep Plasma 114 111% - 116% EDTA Plasma 115 104% - 125% Cit plasma 118 108% - 125% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human ALCAM ELISA Kit (CD166) (ab270205) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALCAM (CD166) protein in cell culture media, cell lysate, edta plasma, serum, cit plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ALCAM (CD166) with 0.02 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Activated leukocyte cell adhesion molecule (ALCAM), also known as CD166, is a protein encoded by the ALCAM gene in humans. ALCAM is a type I transmembrane glycosylated member of the immunoglobulin superfamily of proteins and is mainly involved in the processes of cell adhesion and migration. This protein forms a homo-dimer through the extracellular domain portion or can form a hetero-dimer with T-cell differentiation antigen CD6 with the same region of ALCAM, and the affinity for CD6 is higher than self-association. Interaction with CD6 mediates promotes T-cell activation and proliferation, as well as the formation and maturation of the immunological synapse. ALCAM also mediates attachment of dendritic cells onto endothelial cells by homotypic interaction.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human ALCAM (CD166) Capture Antibody 1 x 600µl 10X Human ALCAM (CD166) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human ALCAM (CD166) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Database links
- SwissProt: Q13740 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human ALCAM standard curve in Sample Diluent NS.The ALCAM standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ALCAM in human serum, plasma (EDTA), plasma (citrate), plasma (heparin).The concentrations of ALCAM were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 3.75%, plasma (EDTA) 2.5%, plasma (citrate) 5%, and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 94.3 ng/mL in serum, 73.1 ng/mL in plasma (EDTA), 112.0 ng/mL in plasma (citrate), and 82.9 ng/mL in plasma (heparin).
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Interpolated concentrations of native ALCAM in human HT-1080 cell extract based on a 250 µg/mL extract load.The concentrations of ALCAM were measured in duplicate and interpolated from the ALCAM standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Serum from eight individual healthy ALCAM gender donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALCAM concentration was determined to be 111 ng/mL with a range of 86 – 133 ng/mL.
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Sandwich ELISA - Human ALCAM ELISA Kit (CD166) (ab270205)To learn more about the advantages of recombinant antibodies see here.
