Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cadherin 8 antibody (ab97268)

Key features and details

  • Rabbit polyclonal to Cadherin 8
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Cadherin 8 antibody
    See all Cadherin 8 primary antibodies

  • Description

    Rabbit polyclonal to Cadherin 8

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: SHSY5Y; WERI; U87MG; SKNBE.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab97268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 88 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • Western blot - Anti-Cadherin 8 antibody (ab97268)
    Western blot - Anti-Cadherin 8 antibody (ab97268)
    All lanes : Anti-Cadherin 8 antibody (ab97268) at 1 µg/ml

    Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 2 : WERI (Human Retinoblastoma) Whole Cell Lysate
    Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 4 : SK N BE (Human neuroblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 88 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute


    Cadherin 8 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Immunocytochemistry/ Immunofluorescence - Anti-Cadherin 8 antibody (ab97268)
    Immunocytochemistry/ Immunofluorescence - Anti-Cadherin 8 antibody (ab97268)
    ICC/IF image of ab97268 stained U87MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97268, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References (1)

Publishing research using ab97268? Please let us know so that we can cite the reference in this datasheet.

ab97268 has been referenced in 1 publication.

  • Mills F  et al. Cadherins mediate cocaine-induced synaptic plasticity and behavioral conditioning. Nat Neurosci 20:540-549 (2017). PubMed: 28192395

Images

  • Western blot - Anti-Cadherin 8 antibody (ab97268)
    Western blot - Anti-Cadherin 8 antibody (ab97268)
    All lanes : Anti-Cadherin 8 antibody (ab97268) at 1 µg/ml

    Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 2 : WERI (Human Retinoblastoma) Whole Cell Lysate
    Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 4 : SK N BE (Human neuroblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 88 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute


    Cadherin 8 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Immunocytochemistry/ Immunofluorescence - Anti-Cadherin 8 antibody (ab97268)
    Immunocytochemistry/ Immunofluorescence - Anti-Cadherin 8 antibody (ab97268)
    ICC/IF image of ab97268 stained U87MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97268, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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