Anti-MAPRE1/EB1 antibody (ab232970)
Key features and details
- Rabbit polyclonal to MAPRE1/EB1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-MAPRE1/EB1 antibody
See all MAPRE1/EB1 primary antibodies -
Description
Rabbit polyclonal to MAPRE1/EB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow, Orangutan
-
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human MAPRE1/EB1 aa 1 to the C-terminus. Expressed in E.coli.
Database link: Q15691 -
Positive control
- WB: Recombinant human MAPRE1/EB1 protein; HeLa and Jurkat cell lysates; Pig brain lysate. IHC-P: Human liver and kidney tissue. ICC/IF: HeLa cells.
-
General notes
This product was previously labelled as MAPRE1
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-MAPRE1/EB1 antibody (ab232970)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MAPRE1/EB1 (green) using ab232970 at 20 μg/ml in ICC/IF. FITC labeling.
-
Western blot - Anti-MAPRE1/EB1 antibody (ab232970)All lanes : Anti-MAPRE1/EB1 antibody (ab232970) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 3 : Pig brain lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 30 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPRE1/EB1 antibody (ab232970)Formalin-fixed, paraffin-embedded human liver tissue stained for MAPRE1/EB1 using ab232970 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPRE1/EB1 antibody (ab232970)Formalin-fixed, paraffin-embedded human kidney tissue stained for MAPRE1/EB1 using ab232970 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Western blot - Anti-MAPRE1/EB1 antibody (ab232970)Anti-MAPRE1/EB1 antibody (ab232970) at 3 µg/ml + Recombinant human MAPRE1/EB1 protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 30 kDa
