Anti-MAPRE1/EB1 antibody (ab50188)
Key features and details
- Rabbit polyclonal to MAPRE1/EB1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MAPRE1/EB1 antibody
See all MAPRE1/EB1 primary antibodies -
Description
Rabbit polyclonal to MAPRE1/EB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Pig
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Immunogen
Synthetic peptide corresponding to Human MAPRE1/EB1 aa 252-268 (C terminal) conjugated to keyhole limpet haemocyanin.
Sequence:GFVIPDEGGPQEEQEEY
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Positive control
- Cell lysate of the rat fibroblast cell line Rat1, Rat1 cells and a HeLa cell lysate.
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General notes
This product was previously labelled as MAPRE1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab50188 is affinity-purified using the immunizing peptide immobilized on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MAPRE1/EB1 antibody (ab50188)ab50188 at 1ug/ml dilution staining the “plus-end tracking protein” (+TIP) structures in Rat1 fibroblasts grown to semi-confluency and fixed with acetone/methanol. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate. Antibody dilution 1:1,000.
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Western blot - Anti-MAPRE1/EB1 antibody (ab50188)All lanes : Anti-MAPRE1/EB1 antibody (ab50188) at 0.5 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : NIH3T3 cell lysate
Lane 3 : Rat1 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : SW48 cell lysate
Lane 6 : HCT116 cell lysate
Lane 7 : HEK293 cell lysate
Lane 8 : HeLa cell lysate with EB1 immunizing peptide at 10 µg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
50kDa band represents Rb IgG heavy chain -
Immunoprecipitation - Anti-MAPRE1/EB1 antibody (ab50188)RIPA lysate of HeLa cells immunoprecipitated with ab50188, separated SDS-PAGE and blotted with ab50188. 50kDa band represents Rb IgG heavy chain
Lane 1 : No primary antibody
Lanes 2-4 : Anti-MAPRE1/EB1 antibody (ab50188) at 15 µg
Lanes 1-2 : No lysate
Lane 3 : HeLa cell lysate at 100 µg
Lane 4 : HeLa cell lysate at 25 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
