Cathepsin G Activity Assay Kit (Colorimetric) (ab126780)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell Lysate, Serum, Tissue Extracts
Overview
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Product name
Cathepsin G Activity Assay Kit (Colorimetric)
See all Cathepsin G kits -
Detection method
Colorimetric -
Sample type
Serum, Tissue Extracts, Cell Lysate -
Assay type
Enzyme activity (quantitative) -
Range
0 nmol/well - 50 nmol/well -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Cathepsin G Activity Assay Kit (Colorimetric) (ab126780) is an assay where Cathepsin G will cleave the substrate and release the dye group, p-NA (4-Nitroaniline), which can be detected at 405 nm. In presence of the Cathepsin G specific inhibitor, the cleavage will be stopped. The kit provides a rapid, simple, sensitive, and reliable test for the activity of Cathepsin G.
Visit our FAQs page for tips and troubleshooting.
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Notes
Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. The protein is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Assay Buffer WM 1 x 25ml Cathepsin G Inhibitor Blue 1 x 20µl p-NA Standard (0.1 M) Yellow 1 x 20µl Positive Control Green 1 vial Substrate Red 1 x 200µl -
Research areas
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Function
Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Cellular localization
Cell surface. - Information by UniProt
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Alternative names
- CATG
- CATG_HUMAN
- Cathepsin G
see all
Images
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Functional Assay: ab126780 Cathepsin G Activity Assay Kit (Colorimetric)
Standard curve with background signal subtracted (duplicates; +/- SD).
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Functional Assay: ab126780 Cathepsin G Activity Assay Kit (Colorimetric)Cathepsin G activity in THP-1 lysates (duplicates +/- SD). Data corresponds to 1 million cells per well.
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Functional Studies - Cathepsin G Activity Assay Kit (ab126780)p-NA Standard Curve. Performed according to assay protocol. -
Functional Studies - Cathepsin G Activity Assay Kit (ab126780)Assay results for 5 μl of positive control with and without an inhibitor
