Cathepsin G Inhibitor Screening Assay Kit (Colorimetric) (ab204693)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Overview
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Product name
Cathepsin G Inhibitor Screening Assay Kit (Colorimetric)
See all Cathepsin G kits -
Detection method
Colorimetric -
Sample type
Inhibitor compounds -
Assay type
Enzyme activity -
Product overview
Cathepsin G Inhibitor Screening Assay Kit (Colorimetric) (ab204693) uses the ability of active Cathepsin G to cleave a synthetic pNA (4-Nitroaniline)-based peptide substrate to release pNA, which can be easily quantified using a colorimetric microplate reader. In the presence of a Cathepsin G inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the pNA absorbance. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin G.
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Notes
Cathepsin G (CTSG, EC 3.4.21.20) is a Serine protease found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Cathepsin G (human) 1 vial CTSG Assay Buffer 1 x 20ml CTSG Inhibitor (0.5 mM) 1 x 20µl CTSG Substrate 1 x 200µl -
Research areas
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Function
Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Cellular localization
Cell surface. - Information by UniProt
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Alternative names
- CATG
- CATG_HUMAN
- Cathepsin G
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Images
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Cathepsin G Inhibitor Screening Assay Kit (Colorimetric) (ab204693)
Inhibition of Cathepsin G activity by CTSG Inhibitor.
