Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free (ab207550)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3056Y] to Cathepsin D - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free
    See all Cathepsin D primary antibodies

  • Description

    Rabbit monoclonal [EPR3056Y] to Cathepsin D - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431, HepG2 and MCF7 whole cell lysate. IHC-P: Human thyroid cancer tissue. ICC: MCF-7 cells. Flow Cyt: MCF-7 cells.

  • General notes

    ab207550 is the carrier-free version of ab75811 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab207550 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free (ab207550)
    Western blot - Anti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free (ab207550)
    Anti-Cathepsin D antibody [EPR3056Y] (ab75811) at 1/1000 dilution + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Predicted band size: 46 kDa



    This data was developed using ab75811, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free (ab207550)
    Western blot - Anti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free (ab207550)
    All lanes : Anti-Cathepsin D antibody [EPR3056Y] (ab75811) at 1/1000 dilution (Unpurified)

    Lane 1 : Wild-type A431 whole cell lysate
    Lane 2 : CTSD knockout A431 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 46 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab75811 observed at 44 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab75811 was shown to specifically react with in wild-type A431 cells as signal was lost in CTSD knockout cells. Wild-type and CTSD knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab75811 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75811).

  • Immunocytochemistry - Anti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free (ab207550)
    Immunocytochemistry - Anti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free (ab207550)
    This data was developed using ab75811, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Cathepsin D with purified ab75811 at 1/50 dilution (1.88 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free (ab207550)
    Flow Cytometry - Anti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free (ab207550)
    This data was developed using ab75811, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Cathepsin D with purified ab75811 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free (ab207550)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free (ab207550)
    This data was developed using ab75811, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue sections labeling Cathepsin D with purified ab75811 at 1/100 dilution (0.94 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Anti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free (ab207550)
    Anti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free (ab207550)

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