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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules Tubulin

Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)

Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16778] to beta I Tubulin - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Monkey

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Overview

  • Product name

    Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free
    See all beta I Tubulin primary antibodies
  • Description

    Rabbit monoclonal [EPR16778] to beta I Tubulin - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250104 is the carrier-free version of ab179511. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250104 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16778
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin

Images

  • Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/20000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 4 : 293T (Human epithelial cells from embryonic kidney) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    This data was developed using ab179511, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    This data was developed using ab179511, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    This data was developed using ab179511, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    This data was developed using ab179511, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    Western blot - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/1000 dilution

    Lane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates
    Lane 2 : BHK (Hamster kidney fibroblast cells) whole cell lysates
    Lane 3 : MDCK (Canine kidney cell line) whole cell lysates
    Lane 4 : MDBK (Bovine kidney cell line) whole cell lysates
    Lane 5 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    This data was developed using ab179511, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    Immunocytochemistry - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    This data was developed using ab179511, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling beta I Tubulin with ab179511 at 1/500 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/400 dilution (green). Cytoplasm and nuclear staining on K562 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab179511 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    This data was developed using ab179511, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on germinal center lymphocytes of Human tonsil is observed. Counter stained with Hematoxylin. Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    This data was developed using ab179511, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on neuron cells of Mouse cerebral cortex tissue is observed. Counter stained with Hematoxylin. Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    This data was developed using ab179511, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on follicular center lymphocytes of Rat spleen tissue is observed. Counter stained with Hematoxylin. Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    Flow Cytometry - Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    This data was developed using ab179511, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling beta I Tubulin with ab179511 at 1/80 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
  • Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
    Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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