Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1491Y] to PKC zeta (phospho T410) + PKC lambda (phospho T412) - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free
    See all PKC zeta (phospho T410) + PKC lambda (phospho T412) primary antibodies

  • Description

    Rabbit monoclonal [EP1491Y] to PKC zeta (phospho T410) + PKC lambda (phospho T412) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects PKC zeta and PKC lamda phosphorylated at T410/412 respectively.

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247446 is the carrier-free version of ab76129. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247446 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)
    Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)
    Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution (purified) + Mouse lung tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 74 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76129, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)

    This data was developed using ab76129, the same antibody clone in a different buffer formulation.

    Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)
    Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)
    All lanes : Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/2000 dilution (unpurified)

    Lane 1 : 293 cell lysates, untreated
    Lane 2 : 293 cell lysates, treated with AP

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit-HRP at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa
    Additional bands at: 165 kDa, 170 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab76129, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)
    This data was developed using ab76129, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded mouse kidney with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)
    This data was developed using ab76129, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human endometrium with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)
    Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)
    All lanes : Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution (purified)

    Lane 1 : Untreated HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 68 kDa
    Additional bands at: 74 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab76129, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)
    This data was developed using ab76129, the same antibody clone in a different buffer formulation.Immunohistochemical staining of PKC zeta in human brain with unpurified ab76129 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)
    Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - BSA and Azide free (ab247446)

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