Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (ab268199)

Key features and details

Mouse monoclonal [KRT15/2958] to Cytokeratin 15 - BSA and Azide free
Suitable for: Flow Cyt, WB, IHC-P, Protein Array
Reacts with: Human
Isotype: IgG2b

Product name

Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free
See all Cytokeratin 15 primary antibodies
Description

Mouse monoclonal [KRT15/2958] to Cytokeratin 15 - BSA and Azide free
Host species

Mouse
Tested applications

Suitable for: Flow Cyt, WB, IHC-P, Protein Arraymore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant full length protein within Human Cytokeratin 15. The exact sequence is proprietary.
Database link: P19012
Positive control
- WB: Human thymus lysate. IHC-P: Human prostate carcinoma tissue. Flow Cyt: HeLa cells.
General notes

ab268199 is the PBS only version of ab268078.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from bioreactor concentrate.
Clonality

Monoclonal
Clone number

KRT15/2958
Isotype

IgG2b
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (ab268199)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Cytokeratin 15 using ab268078 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)
Flow Cytometry - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (ab268199)

Flow cytometric analysis of PFA-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Cytokeratin 15 using ab268078 at 2 μg/million cells followed by a goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)
Western blot - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (ab268199)

Anti-Cytokeratin 15 antibody [KRT15/2958] (ab268078) at 2 µg/ml + Human thymus lysate

Predicted band size: 49 kDa

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (ab268199)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Cytokeratin 15 using ab268078 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)
Protein Array - Anti-Cytokeratin 15 antibody [KRT15/2958] - BSA and Azide free (ab268199)

Protein Array containing more than 19,000 full-length human proteins using ab268078.

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268078)

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