Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (ab248375)
![Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (ab248375)](https://www.abcam.com/ps/products/248/ab248375/Images/ab248375-405732-anti-acat2-acetyl-coa-acetyltransferase-antibody-epr8417-bsa-and-azide-free-western-blot.jpg "Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (ab248375)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8417] to ACAT2/Acetyl-CoA acetyltransferase - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free
See all ACAT2/Acetyl-CoA acetyltransferase primary antibodies -
Description
Rabbit monoclonal [EPR8417] to ACAT2/Acetyl-CoA acetyltransferase - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248375 is the carrier-free version of ab131215 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248375 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as ACAT2, Acetyl-CoA acetyltransferase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.91 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR8417 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
Images
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Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (ab248375)All lanes : Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 41 kDaThis data was developed using ab131215, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (ab248375)](https://www.abcam.com/ps/products/248/ab248375/Images/ab248375-2-anti-acat2-acetyl-coa-acetyltransferase-antibody-epr8417-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (ab248375)This data was developed using ab131215, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human liver tissue labelling ACAT2/Acetyl-CoA acetyltransferase with ab131215 antibody at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
![OI-RD Scanning - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (ab248375)](https://www.abcam.com/ps/products/248/ab248375/Images/ab248375-1-anti-acat2-acetyl-coa-acetyltransferase-antibody-epr8417-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (ab248375)This data was developed using ab131215, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
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![Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (ab248375)](https://www.abcam.com/ps/products/248/ab248375/Images/ab248375-4-benefits-of-recombinant-antibodies.png)
Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] - BSA and Azide free (ab248375)