Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14222] to PHF1 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PHF1 antibody [EPR14222] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR14222] to PHF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human pancreas tissue.

  • General notes

    Ab232601 is the carrier-free version of ab184951. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232601 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14222

  • Isotype

    IgG

Images

  • Flow Cytometry - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)
    Flow Cytometry - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)

    Flow Cytometric analysis of 293 cells (2% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/160 dilution (red) mpared to a negative control antibody (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184951).

  • Immunocytochemistry/ Immunofluorescence - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)
    Immunocytochemistry/ Immunofluorescence - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)

    Immunofluorescent analysis of MCF7 cells (4% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184951).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184951).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184951).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)
    Anti-PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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