Antibodies, Proteins, Kits and Reagents for Life Science

365 193 ₸ Product size

100 µl 365 193 ₸ 10 µl 150 768 ₸

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Anti-LDL Receptor antibody [EP1553Y] (ab52818)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1553Y] to LDL Receptor
Suitable for: IHC-P, WB
Reacts with: Mouse, Human

Product name

Anti-LDL Receptor antibody [EP1553Y]
See all LDL Receptor primary antibodies
Description

Rabbit monoclonal [EP1553Y] to LDL Receptor
Host species

Rabbit
Specificity

Some optimisation may be required for detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for suitable positive controls.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human LDL Receptor aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P01130
Positive control
- WB: HeLa and RAW264.7 cell lysate. HepG2 whole cell lysate. Mouse liver, lung and colon lysate. Human liver and plasma lysate. IHC-P: Human hepatocellular carcinoma and liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1553Y
Isotype

IgG
Research areas

Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)

All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution

Lane 1 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
Lane 3 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 5 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 7 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 95 kDa
Observed band size: 150 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST

Diluting buffer: 5% NFDM/TBST
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)

All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa treated with GW3965 for 8 hours at the final concentration of 5uM whole cell lysates
Lane 3 : HeLa treated with GW3965 for 24 hours at the final concentration of 5uM whole cell lysates
Lane 4 : Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysates
Lane 5 : Raw264.7 treated with GW3965 for 8 hours at the final concentration of 5uM whole cell lysates
Lane 6 : Raw264.7 treated with GW3965 for 24 hours at the final concentration of 5uM whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

Predicted band size: 95 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking buffer: 5% NFDM/TBST

Diltuion buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)

Immunohistochemical analysis of paraffin-embedded human liver sections labeling LDL Receptor with purified ab52818 at dilution of 1:500. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)

Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 0.4 µg/ml + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 95 kDa

Exposure time: 3 minutes

Blocking and diluting buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with the literature (PMID: 15199428, PMID: 8349823, PMID: 10906332, PMID: 24918045).
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)

All lanes : Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/1000 dilution

Lane 1 : Mouse liver lysate
Lane 2 : Mouse lung lysate
Lane 3 : Mouse colon lysate
Lane 4 : Human liver lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (HRP goat anti-rabbit IgG (H+L))

Predicted band size: 95 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking buffer: 5% NFDM/TBST

Diltuion buffer: 5% NFDM/TBST
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)

Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/5000 dilution + Mouse liver at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Predicted band size: 95 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Blocking/Diluting buffer 5% NFDM/TBST
Western blot - Anti-LDL Receptor antibody [EP1553Y] (ab52818)

Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/500 dilution + Human plasma total protein lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 95 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 27 kDa, 48 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 4 minutes

LDL Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [EP1553Y] (ab52818)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling LDL Receptor with ab52818 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counterstained with hematoxylin.
Anti-LDL Receptor antibody [EP1553Y] (ab52818)

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