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Anti-LDL Receptor antibody [1B10H10] (ab204941)

Key features and details

  • Mouse monoclonal [1B10H10] to LDL Receptor
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-LDL Receptor antibody [1B10H10]
    See all LDL Receptor primary antibodies

  • Description

    Mouse monoclonal [1B10H10] to LDL Receptor

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human LDL Receptor aa 1-150. (Purified from E.coli).
    Database link: P01130

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant Human LDL Receptor (aa22-150) protein; LDL Receptor (aa22-150)-hIgGFc transfected HEK293 cell lysate; Human ovarian cancer tissue; HeLa cells.

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR299695-5 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-LDL Receptor antibody [1B10H10] (ab204941)
    Western blot - Anti-LDL Receptor antibody [1B10H10] (ab204941)
    Anti-LDL Receptor antibody [1B10H10] (ab204941) at 1/500 dilution + Recombinant Human LDL Receptor (aa22-150) protein

    Predicted band size: 95 kDa



    Expected MWt is 40.1 kDa.

  • Flow Cytometry - Anti-LDL Receptor antibody [1B10H10] (ab204941)
    Flow Cytometry - Anti-LDL Receptor antibody [1B10H10] (ab204941)

    Flow cytometric analysis of HeLa cells labeling LDL Receptor with ab204941 at 1/200 dilution (green), compared to a negative control (red). Cells were fixed with 4% paraformaldehyde and incubated at 4°C for 30 minutes and permeabilized with 0.5 mL filter buffer (0.1%Triton-X100 and Blocking buffer) and incubated at 4°C for 15 minutes.
    Alexa Fluor 488, goat anti-mouse IgG (H+L) at 1/200 was used as the secondary antibody.

  • Western blot - Anti-LDL Receptor antibody [1B10H10] (ab204941)
    Western blot - Anti-LDL Receptor antibody [1B10H10] (ab204941)
    All lanes : Anti-LDL Receptor antibody [1B10H10] (ab204941) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : LDL Receptor (aa22-150)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 95 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [1B10H10] (ab204941)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody [1B10H10] (ab204941)

    Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling LDL Receptor with ab204941 at 1/200 dilution with DAB staining.

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