Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BCL2L10 antibody [8A2F9] (ab233641)

Key features and details

  • Mouse monoclonal [8A2F9] to BCL2L10
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-BCL2L10 antibody [8A2F9]
    See all BCL2L10 primary antibodies

  • Description

    Mouse monoclonal [8A2F9] to BCL2L10

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human BCL2L10 aa 1 to the C-terminus. (Expressed in E.coli).
    Database link: Q9HD36

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: BCL2L10 (aa 31-186)-hIgGFc transfected HEK-293 cell lysate; Human BCL2L10 (aa 31-186) recombinant protein. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. IHC-P: Human lung cancer and cervical cancer tissues.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-BCL2L10 antibody [8A2F9] (ab233641)
    Western blot - Anti-BCL2L10 antibody [8A2F9] (ab233641)
    Anti-BCL2L10 antibody [8A2F9] (ab233641) at 1/500 dilution + Human BCL2L10 (aa 31-186) recombinant protein

    Predicted band size: 22 kDa



    Expected MW is 43.1 kDa.

  • Western blot - Anti-BCL2L10 antibody [8A2F9] (ab233641)
    Western blot - Anti-BCL2L10 antibody [8A2F9] (ab233641)
    All lanes : Anti-BCL2L10 antibody [8A2F9] (ab233641) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : BCL2L10 (aa 31-186)-hIgGFc transfected HEK-293 cell lysate

    Predicted band size: 22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-BCL2L10 antibody [8A2F9] (ab233641)
    Immunocytochemistry/ Immunofluorescence - Anti-BCL2L10 antibody [8A2F9] (ab233641)

    HeLa  (human epithelial cell line from cervix adenocarcinoma) cells stained for BCL2L10 (green) using ab233641 at 1/200 dilution in ICC/IF.

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Flow Cytometry - Anti-BCL2L10 antibody [8A2F9] (ab233641)
    Flow Cytometry - Anti-BCL2L10 antibody [8A2F9] (ab233641)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling BCL2L10 with ab233641 at 1/200 dilution (green) compared with a negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L10 antibody [8A2F9] (ab233641)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L10 antibody [8A2F9] (ab233641)

    Paraffin-embedded human lung cancer tissue stained for BCL2L10 using ab233641 at 1/200 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L10 antibody [8A2F9] (ab233641)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L10 antibody [8A2F9] (ab233641)

    Paraffin-embedded human cervical cancer tissue stained for BCL2L10 using ab233641 at 1/200 dilution in immunohistochemical analysis.

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