Antibodies, Proteins, Kits and Reagents for Life Science

Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (ab280895)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24290-356] to SOCS1 - BSA and Azide free
Suitable for: IP, WB, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free
See all SOCS1 primary antibodies
Description

Rabbit monoclonal [EPR24290-356] to SOCS1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IP, WB, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivity

Reacts with: Mouse, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: KARPAS-299, K562, Mouse thymus lysates. Flow Cyt: KARPAS-299 cells. IP: KARPAS-299 cells.
General notes
ab280895 is the carrier-free version of ab280886. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab280895 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24290-356
Isotype

IgG
Research areas

Western blot - Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (ab280895)

All lanes : Anti-SOCS1 antibody [EPR24290-356] (ab280886) at 1/1000 dilution

Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 3 : U266B1 (human multiple myeloma B lymphocyte) whole cell lysate

Lysates/proteins at 60 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 23 kDa
Observed band size: 23,22 kDa
why is the actual band size different from the predicted?

This data was developed using ab280886, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:15580314, 26090662, 10490100).

Negative control: U266B1 (PMID: 15580314).

Exposure time: Lane 1: 48 seconds Lane 2-3: 3 minutes
Flow Cytometry - Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (ab280895)

This data was developed using ab280886, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U266B1 (human multiple myeloma B lymphocyte, left)/ KARPAS-299 (Human anaplastic large cell lymphoma, right) cells labelling SOCS1 with ab280886 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: U266B1 (PMID: 15580314).
Immunoprecipitation - Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (ab280895)

This data was developed using ab280886, the same antibody clone in a different buffer formulation.

SOCS1 was immunoprecipitated from 0.35 mg of KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate with ab280886 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280886 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10 ug

Lane 2: ab280886 IP in KARPAS-299 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280886 in mouse eyeball tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 93 seconds
Western blot - Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (ab280895)

Anti-SOCS1 antibody [EPR24290-356] (ab280886) at 1/1000 dilution + Mouse thymus tissue lysate at 60 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 23 kDa
Observed band size: 23 kDa

This data was developed using ab280886, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 15580314, 26090662, 10490100).

Exposure time: 3 minutes
Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (ab280895)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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