Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker (ab9021)
Key features and details
- Mouse monoclonal [RCK105] to Cytokeratin 7 - Cytoskeleton Marker
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 7 antibody [RCK105] - Cytoskeleton Marker
See all Cytokeratin 7 primary antibodies -
Description
Mouse monoclonal [RCK105] to Cytokeratin 7 - Cytoskeleton Marker -
Host species
Mouse -
Specificity
This antibody reacts exclusively with cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr HumanPigIHC-P Human -
Immunogen
Cytokeratins from the human bladder carcinoma cell line T24.
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Positive control
- IHC-P: Human liver tissue, mouse ovary and bladder tissue IHC-Fr: Human liver, urinary bladder tissue, Pig liver and skin tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
RCK105 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry analysis of formalin fixed, paraffin embedded sections of human liver tissue labeling Cytokeratin 7 with ab9021 at 1/100; showing positive staining in the epithelial cells lining the bile ducts and no reactivity in hepatocytes or connective tissue.
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ab9021 staining normal pig skin tissue section (frozen), staining epithelial cells using ab9021 at 1/500.
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Immunofluorescent staining of β-galactosideasae and cytokeratin expression of urinary bladder after intravesical treatment with Adeno-Cre
Formalin-fixed, paraffin-embedded mouse bladder tissue stained for β-galactosidase (green, A) or Cytokeratin 7 (red, B). Panel C shows the colocalization of β-galactosidase and Cytokeratin 7. The nuclear counterstain is DAPI (blue).
Cytokeratin 7 was detected using ab9021.
(After Figure 3 of Yang et al)
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ab9021 staining normal breast tissue section (frozen), fixated in Methanol, staining epithelial cells. These pictures are part of the review submitted by Rene Villadsen.
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Immunohistochemistry analysis of frozen sections of human liver labeling Cytokeratin 7 with ab9021 at 1/500; showing positive staining in the epithelial cells lining the bile ducts and no reactivity in hepatocytes or connective tissue.
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Immunohistochemistry analysis of frozen sections of human urinary bladder labeling Cytokeratin 7 with ab9021 at 1/500; showing positive staining in the epithelial cells and no reactivity in connective tissue.
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Immunohistochemistry on frozen sections of human liver, only bile duct epithelium positive; no reaction in hepatocytes
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Immunohistochemistry on frozen sections of swine liver, only bile duct epithelium positive; no reaction in hepatocytes
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Formalin fixed parrafin embedded mouse ovary tissue. Ab9021 used at 1:1000 incubated with overnight incubation with a biotinylated secondary and peroxidase/DAB detection.