Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat skin tissue sections labeling Cytokeratin 5 with ab64081 at 1/100 dilution (2.46 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on rat skin, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab64081 for 30 mins at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse skin tissue sections labeling Cytokeratin 5 with ab64081 at 1:100 dilution (2.46 ?g/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on mouse skin, performed on a Leica Biosystems BOND^TM RX instrument
The section was incubated with ab64081 for 30 mins at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung squamous cell cancer tissue sections labeling Cytokeratin 5 with ab64081 at 1/100 dilution (2.46 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on tumor cells of human lung squamous cell cancer, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab64081 for 30 mins at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate cancer
tissue sections labeling Cytokeratin 5 with ab64081 at 1/100 dilution (2.46 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human prostate cancer, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab64081 for 30 mins at room temperature.

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Frozen) analysis of mouse skin tissue section labeling Cytokeratin 5 with purified ab64081 at 1/30 (8.2 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 5 with purified ab64081 at 1/100 (2.5 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Frozen) analysis of rat skin tissue section labeling Cytokeratin 5 with purified ab64081 at 1/30 (8.2 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Anti-Cytokeratin 5 antibody [SP27] (ab64081) at 1/100 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate

Predicted band size: 62 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?

Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling Cytokeratin 5 with ab64081 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human thymus tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human skin tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human lung tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human breast tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human bladder tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Cytokeratin 5 using ab64081 at 1/100 dilution in immunohistochemical analysis.