Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1600Y] to Cytokeratin 5
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 5 antibody [EPR1600Y]
    See all Cytokeratin 5 primary antibodies

  • Description

    Rabbit monoclonal [EPR1600Y] to Cytokeratin 5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Cytokeratin 5.
    Database link: P13647

  • Positive control

    • A431 cell lysate and human squamous cervical carcinoma tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 3.19 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR1600Y

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)

    Fluorescent immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using unpurified ab75869. Green-CK5 red-PI

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)

    Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma) cells labelling Cytokeratin 5 with purified ab75869 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei couterstained with DAPI (blue).

    For negative control 1, rabbit primary antibody was used, followed by anti-mouse secondary antibody (ab150120). For negative control 2, mouse primary antibody (ab7291) was used followed by anti-rabbit secondary antibody (ab150077).  

  • Western blot - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)
    Western blot - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)
    All lanes : Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869) at 1/50000 dilution (purified)

    Lane 1 : A431 (human epidermoid carcinoma) whole cell lysate
    Lane 2 : HACAT (human keratinocyte) whole cell lysate
    Lane 3 : Human skin tissue lysate
    Lane 4 : Human fetal tonsil tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa



    Blocking and diluting buffer 5% NFDM/TBST
  • Flow Cytometry - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)
    Flow Cytometry - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)

    Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Cytokeratin 5 with purified ab75869 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • Western blot - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)
    Western blot - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)
    Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869) at 1/10000 dilution (unpurified) + A431 cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)

    Immunohistochemical analysis of paraffin embedded human tonsil tissue section labelling Cytokeratin 5 with purified ab75869 at dilution of 1/1000. The secondary antibody used was HRP-conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)

    Unpurified ab75869 at 1/100 dilution staining Cytokeratin 5 in human squamous cervical carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)

    Fluorescent immunohistochemical analysis of paraffin-embedded human cervical carcinoma using unpurified ab75869. Green-CK5 red-PI.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)
    OI-RD Scanning - Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)
    Anti-Cytokeratin 5 antibody [EPR1600Y] (ab75869)

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