Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17079] to Cytokeratin 7 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free
    See all Cytokeratin 7 primary antibodies

  • Description

    Rabbit monoclonal [EPR17079] to Cytokeratin 7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251287 is the carrier-free version of ab199718. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251287 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)

    This data was developed using ab199718, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse lung tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)
    Western blot - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)
    All lanes : Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/20000 dilution

    Lane 1 : Mouse lung tissue lysate
    Lane 2 : Mouse bladder tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 45,51 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab199718, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    ~45 kDa bands are supported by the literature and competitor's products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID: 16265353.

  • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)
    Western blot - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)
    Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/20000 dilution + Mouse skin tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 45,51 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab199718, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    ~45 kDa bands are supported by the literature and competitor's products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID: 16265353.

  • Western blot - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)
    Western blot - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)
    All lanes : Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/4000 dilution

    Lane 1 : Rat bladder tissue lysate
    Lane 2 : Rat lung tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 45,51 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab199718, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    ~45 kDa bands are supported by the literature and competitor's products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID: 16265353.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)
    This data was developed using ab199718, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse colon tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)
    Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)

    This data was developed using ab199718, the same antibody clone in a different buffer formulation.

    Cytokeratin 7 was immunoprecipitated from 1mg of Mouse skin whole cell extract with ab199718 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199718 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Mouse skin whole cell extract 10 µg (Input). Lane 2: ab199718 IP in Mouse skin whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199718 in Mouse skin whole cell extract.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)
    Anti-Cytokeratin 7 antibody [EPR17079] - BSA and Azide free (ab251287)

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