Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [LP2K] to Cytokeratin 19 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free
    See all Cytokeratin 19 primary antibodies

  • Description

    Mouse monoclonal [LP2K] to Cytokeratin 19 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICCmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • WB: Human skin, kidney and stomach tissue lysate. HepG2, MCF7 and HT-29 whole cell lysate. IHC-P: Human lung carcinoma tissue. Human kidney tissue. ICC: MCF7 cells. Flow Cyt: MCF7 cells.

  • General notes

    ab255939 is the carrier-free version of ab254186.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)
    Western blot - Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)
    All lanes : Anti-Cytokeratin 19 antibody [LP2K] (ab254186) at 1/2000 dilution

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human colon tissue lysate
    Lane 3 : Human stomach tissue lysate
    Lane 4 : Human spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 44 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab254186, the same antibody clone in a different buffer formulation.

    The expression profile observed is consistent with what has been described in the literature (PMID: 24309898).
    Negative control: human spleen (PMID: 24309898).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)

    This data was developed using ab254186, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling Cytokeratin 19 with ab254186 at 1/10000 (0.105 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human lung carcinoma is observed. The section was incubated with ab254186 for 30 mins at room temperature and followed by specific mouse IgG antibody (ab190482) for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunocytochemistry - Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)
    Immunocytochemistry - Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)

    This data was developed using ab254186, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 100% Methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling Cytokeratin 19 with ab254186 at 1/500 (2.096 µg/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in MCF7 cells.

    ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab254186 at a 1/50 dilution followed by ab150080 at a 1/1000 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

  • Flow Cytometry - Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)
    Flow Cytometry - Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)

    This data was developed using ab254186, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Cytokeratin 19 with ab254186 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse monoclonal IgG  (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)
    Western blot - Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)
    All lanes : Anti-Cytokeratin 19 antibody [LP2K] (ab254186) at 1/2000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : Human skin tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 44 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    This data was developed using ab254186, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 3 secs; Lane 4: 48 secs.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)

    This data was developed using ab254186, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Cytokeratin 19 with ab254186 at 1/10000 (0.105 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human kidney is observed. The section was incubated with ab254186 for 30 mins at room temperature and followed by specific mouse IgG antibody (ab190482) for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)

    This data was developed using ab254186, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Cytokeratin 19 with ab254186 at 1/10000 (0.105 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254186 for 30 mins at room temperature and followed by specific mouse  IgG antibody (ab190482) for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

    Negative control: no staining on human spleen.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)
    Anti-Cytokeratin 19 antibody [LP2K] - BSA and Azide free (ab255939)

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