Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Moesin antibody [EPR2428(2)] - BSA and Azide free (ab246835)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2428(2)] to Moesin - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Moesin antibody [EPR2428(2)] - BSA and Azide free
    See all Moesin primary antibodies

  • Description

    Rabbit monoclonal [EPR2428(2)] to Moesin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Raji, Jurkat and HeLa whole cell lysate (ab150035); Human breast carcinoma tissue; Raji cells.

  • General notes

    Ab246835 is the carrier-free version of ab151542. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab246835 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Moesin antibody [EPR2428(2)] - BSA and Azide free (ab246835)
    Western blot - Anti-Moesin antibody [EPR2428(2)] - BSA and Azide free (ab246835)

    This data was developed using ab151542, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: Moesin knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lane 4: Raji whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab151542 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab151542 was shown to specifically react with Moesin in wild-type HAP1 cells as signal was lost in Moesin knockout cells. Wild-type and Moesin knockout samples were subjected to SDS-PAGE. ab151542 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Moesin antibody [EPR2428(2)] - BSA and Azide free (ab246835)
    Western blot - Anti-Moesin antibody [EPR2428(2)] - BSA and Azide free (ab246835)
    All lanes : Anti-Moesin antibody [EPR2428(2)] (ab151542) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 68 kDa



    This data was developed using ab151542, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2428(2)] - BSA and Azide free (ab246835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [EPR2428(2)] - BSA and Azide free (ab246835)
    This data was developed using ab151542, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Moesin with ab151542 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Moesin antibody [EPR2428(2)] - BSA and Azide free (ab246835)
    Immunocytochemistry - Anti-Moesin antibody [EPR2428(2)] - BSA and Azide free (ab246835)
    This data was developed using ab151542, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of Raji cells labeling Moesin with ab151542 at 1/100 dilution.
  • Anti-Moesin antibody [EPR2428(2)] - BSA and Azide free (ab246835)
    Anti-Moesin antibody [EPR2428(2)] - BSA and Azide free (ab246835)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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