Antibodies, Proteins, Kits and Reagents for Life Science

368 544 ₸ Product size

100 µl 368 544 ₸

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Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1601Y] to Cytokeratin 5 - Cytoskeleton Marker
Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker
See all Cytokeratin 5 primary antibodies
Description

Rabbit monoclonal [EP1601Y] to Cytokeratin 5 - Cytoskeleton Marker
Host species

Rabbit
Specificity

Mouse reactivity is based on IHC (positive tissues: Liver, lung, brain and skin). However, WB was negative for Mouse brain, heart, kidney and spleen. There is background staining in mouse and rat islet.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human Cytokeratin 5 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P13647
Positive control
- WB: A431 cell, human fetal skin, rat skin and mouse skin lysates. IHC-P: Squamous cell cervical, squamous cell lung and basal cell breast carcinoma tissue. Human transitional urinary bladder carcinoma tissue. Normal tonsil squamous, human cervical carcinoma, mouse skin and rat skin tissues. Flow Cytometry and ICC/IF: A431 cells. ICC/IF: A431 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1601Y
Isotype

IgG
Research areas

Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

All lanes : Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/1000 dilution

Lane 1 : Human skin lysates prepared in RIPA lysis method
Lane 2 : Human skin lysates prepared in 1%SDS Hot lysis method
Lane 3 : Mouse skin lysates prepared in RIPA lysis method
Lane 4 : Mouse skin lysates prepared in 1%SDS Hot lysis method

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 62 kDa

The lysates were prepared in 1%SDS Hot lysis method.

Observed MW: 62kDa

Blocking/diluting buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 6 with Purified ab52635 at 1/100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

All lanes : Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/10000 dilution (purified)

Lane 1 : Human fetal skin lysates
Lane 2 : Rat skin lysates
Lane 3 : Mouse skin lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 62 kDa
Observed band size: 62 kDa

Blocking and diluting buffer: 5% NFDM/TBST.

The lysates were prepared in 1%SDS Hot lysis method.
Flow Cytometry - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labelling Cytokeratin 5 with purified ab52635 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor^®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) Khanom, R. et al PLoS One. 2016 Aug 11;11(8):e0161163. doi: 10.1371/journal.pone.0161163. eCollection 2016 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Colocalization of KRT5, KRT6 and KRT17 in HSC3 cells

Immunocytochemistry in HSC3 (human oral squamous carcinoma cell line) cells. Scale bar, 10 μm.

(Taken from Figure S3 of Khanom et al)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat skin tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Unpurified ab52635 showing positive staining in squamous cell cervical carcinoma tissue.
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

A431 cells stained with unpurified ab52635 at 1/100 - 1/250
Western blot - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/10000 dilution (unpurified) + A431 cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 62 kDa
Observed band size: 62 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Human transitional urinary bladder carcinoma stained with unpurified ab52635 at 1/100 - 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Unpurified ab52635 showing positive staining in basal cell breast carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Unpurified ab52635 showing negative staining in ductal breast carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Unpurified ab52635 showing negative staining in stomach adenocarcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Unpurified ab52635 showing positive staining in normal tonsil squamous cells tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

Unpurified ab52635 showing positive staining in squamous cell lung carcinoma tissue.
Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)

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