Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Cytokeratin 20 antibody [EPR1621(2)] (ab109111)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1621(2)] to Cytokeratin 20
Suitable for: Flow Cyt, ICC/IF, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Cytokeratin 20 antibody [EPR1621(2)]
See all Cytokeratin 20 primary antibodies
Description

Rabbit monoclonal [EPR1621(2)] to Cytokeratin 20
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to residues in Human Cytokeratin 20 (P35900).
Positive control
- WB: SW480, Mouse small intestine and Rat small intestine lysates ICC/IF: SW480 cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR1621(2)
Isotype

IgG
Research areas

Flow Cytometry - Anti-Cytokeratin 20 antibody [EPR1621(2)] (ab109111)

Flow Cytometry analysis of SW480 (human colorectal adenocarcinoma ) cells labeling Cytokeratin 20 with unpurified ab109111 at 1:20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Western blot - Anti-Cytokeratin 20 antibody [EPR1621(2)] (ab109111)

All lanes : Anti-Cytokeratin 20 antibody [EPR1621(2)] (ab109111) at 1/10000 dilution

Lane 1 : SW480 lysate
Lane 2 : Mouse small intestine lysate
Lane 3 : Rat small intestine lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 48 kDa
Observed band size: 46 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [EPR1621(2)] (ab109111)

Immunofluorescent staining of Cytokeratin 20 in SW480 cells, using ab109111 at a 1/50 dilution.
Anti-Cytokeratin 20 antibody [EPR1621(2)] (ab109111)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com