Anti-Cytokeratin 20 antibody (ab231954)
Key features and details
- Rabbit polyclonal to Cytokeratin 20
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 20 antibody
See all Cytokeratin 20 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 20 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment corresponding to Human Cytokeratin 20 aa 1 to the C-terminus. Expressed in E.coli.
Database link: P35900 -
Positive control
- IHC-P: Human lung and stomach tissue. WB: Recombinant human Cytokeratin 20 protein; HeLa cell lysate; Pig intestine tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cytokeratin 20 antibody (ab231954)Anti-Cytokeratin 20 antibody (ab231954) at 2 µg/ml + Recombinant human Cytokeratin 20 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 48 kDa
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Western blot - Anti-Cytokeratin 20 antibody (ab231954)All lanes : Anti-Cytokeratin 20 antibody (ab231954) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Pig intestine tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 48 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody (ab231954)Formalin-fixed, paraffin-embedded human lung tissue stained for Cytokeratin 20 using ab231954 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody (ab231954)Formalin-fixed, paraffin-embedded human stomach tissue stained for Cytokeratin 20 using ab231954 at 20 μg/ml in immunohistochemical analysis. DAB staining.
