Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

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Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17079] to Cytokeratin 7
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat

Product name

Anti-Cytokeratin 7 antibody [EPR17079]
See all Cytokeratin 7 primary antibodies
Description

Rabbit monoclonal [EPR17079] to Cytokeratin 7
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse
IP	Mouse
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse lung, bladder and skin lysates; Rat bladder and lung lysates. IHC-P: Mouse lung and colon tissues. IP: Mouse skin whole cell extract .
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17079
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse lung tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

All lanes : Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/20000 dilution

Lane 1 : Mouse lung lysate
Lane 2 : Mouse bladder lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa
Observed band size: 45,51 kDa
why is the actual band size different from the predicted?

Exposure time: 15 seconds

~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.
Blocking/dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/20000 dilution + Mouse skin lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa
Observed band size: 45,51 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

All lanes : Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/4000 dilution

Lane 1 : Rat bladder lysate
Lane 2 : Rat lung lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa
Observed band size: 45,51 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID:16265353.
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse colon tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

Cytokeratin 7 was immunoprecipitated from 1mg of Mouse skin whole cell extract with ab199718 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199718 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Mouse skin whole cell extract 10 µg (Input). Lane 2: ab199718 IP in Mouse skin whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199718 in Mouse skin whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

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