Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸

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Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18862] to GABARAP + GABARAPL1 + GABARAPL3
Suitable for: IP, WB
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862]
See all GABARAP+GABARAPL1+GABARAPL3 primary antibodies
Description

Rabbit monoclonal [EPR18862] to GABARAP + GABARAPL1 + GABARAPL3
Host species

Rabbit

Tested Applications & Species

Application	Species
IP	Mouse
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human GABARAPL1 full length recombinant protein; Recombinant human GABARAPL3 full length protein; Recombinant human GABARAP full length protein; Human brain lysate; HeLa and HepG2 whole cell lysates; Mouse brain, kidney and spleen lysates; Rat brain and kidney lysates. IP: Mouse brain lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18862
Isotype

IgG
Research areas

Western blot - Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888)

Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/1000 dilution + Human GABARAPL1 full length recombinant protein at 0.02 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 14 kDa

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Human GABARAPL1 full length recombinant protein contains aa1-117 with a GST-Tag.
Western blot - Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888)

All lanes : Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/5000 dilution

Lane 1 : Recombinant human GABARAPL2 full length protein
Lane 2 : Recombinant human GABARAPL3 full length protein
Lane 3 : Recombinant human GABARAP full length protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 14 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1 and 2: 5 seconds; Lane 3: 3 minutes.

All three recombinant human full length proteins contain aa1-117 with an N-terminal His-Tag^®. This product showed higher reactivity with GABARAPL1 and GABARAPL3, and lower reactivity with GABARAP.
Western blot - Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888)

Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/1000 dilution + Human brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 14 kDa
Observed band size: 17 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The observed MW is a little higher than predicted one (14KD), which is similar with the published literatures (PMID: 16650615 & 21862879).
Western blot - Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888)

All lanes : Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time:30 seconds

The observed MW is a little higher than predicted one (14KD), which is similar with the published literatures (PMID: 16650615 & 21862879).
Western blot - Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888)

All lanes : Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The observed MW is a little higher than predicted one (14KD), which is similar with the published literatures (PMID: 16650615 & 21862879).
Immunoprecipitation - Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888)

GABARAP + GABARAPL1 + GABARAPL3 was immunoprecipitated from 1 mg of Mouse brain lysate with ab191888 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab191888 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Mouse brain lysate, 10µg (Input).

Lane 2: ab191888 IP in Mouse brain lysate.

Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab191888 in Mouse brain lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888)

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