Anti-GABARAP antibody - N-terminal (ab227732)
Key features and details
- Rabbit polyclonal to GABARAP - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GABARAP antibody - N-terminal
See all GABARAP primary antibodies -
Description
Rabbit polyclonal to GABARAP - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig, Rhesus monkey
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Immunogen
Synthetic peptide within Human GABARAP (N terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: O95166 -
Positive control
- WB: HEK-293T, Jurkat, Raji, NCI-H929, A431 and HeLa whole cell lysates/extracts. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GABARAP antibody - N-terminal (ab227732)All lanes : Anti-GABARAP antibody - N-terminal (ab227732) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GABARAP shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa15% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-GABARAP antibody - N-terminal (ab227732)Ice-cold methanol-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for GABARAP (green) using ab227732 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Western blot - Anti-GABARAP antibody - N-terminal (ab227732)All lanes : Anti-GABARAP antibody - N-terminal (ab227732) at 1/2000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa15% SDS-PAGE gel.
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Western blot - Anti-GABARAP antibody - N-terminal (ab227732)All lanes : Anti-GABARAP antibody - N-terminal (ab227732) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa15% SDS-PAGE gel.
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Western blot - Anti-GABARAP antibody - N-terminal (ab227732)All lanes : Anti-GABARAP antibody - N-terminal (ab227732) at 1/10000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GABARAP whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa15% SDS-PAGE gel.
