Anti-GABARAP antibody - N-terminal (ab228504)
Key features and details
- Rabbit polyclonal to GABARAP - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-GABARAP antibody - N-terminal
See all GABARAP primary antibodies -
Description
Rabbit polyclonal to GABARAP - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Cow
-
Immunogen
Synthetic peptide corresponding to Human GABARAP aa 1-100 (N terminal) (Cysteine residue).
Database link: O95166 -
Positive control
- WB: HEK-293 and Odora cell lysates. ICC/IF: Human white blood cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Lyophilized:Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7 -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-GABARAP antibody - N-terminal (ab228504)
Human white blood cells stained for GABARAP (red) using ab228504 at 1/200 dilution in ICC/IF.
MHC class staining is shown (green). The nuclear counterstain is Hoechst Dye (blue).
The merged image is shown.
-
Western blot - Anti-GABARAP antibody - N-terminal (ab228504)Anti-GABARAP antibody - N-terminal (ab228504) at 1/100 dilution + HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Developed using the ECL technique.
Predicted band size: 13 kDa
-
Western blot - Anti-GABARAP antibody - N-terminal (ab228504)Anti-GABARAP antibody - N-terminal (ab228504) at 1/100 dilution + Odora (human olfactory cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 13 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-GABARAP antibody - N-terminal (ab228504)Human white blood cells stained for GABARAP (red) using ab228504 incubated for 1 hour at room temperature at 1/200 dilution in ICC/IF.
MHC class II staining is shown (green). Nuclear counterstain is Hoechst Dye (blue).
The merged image is shown in panel C.
-
Immunocytochemistry/ Immunofluorescence - Anti-GABARAP antibody - N-terminal (ab228504)Human white blood cells stained for GABARAP (red) using ab228504 at 1/200 dilution in ICC/IF.
-
Immunocytochemistry/ Immunofluorescence - Anti-GABARAP antibody - N-terminal (ab228504)Human white blood cells stained for GABARAP (red) using ab228504 at 1/200 dilution in ICC/IF.
MHC class staining is shown (green). The nuclear counterstain is Hoechst Dye (blue).
The merged image is shown.
