Anti-Alpha1-adaptin antibody [EPR7572] (ab151720)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7572] to Alpha1-adaptin
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Alpha1-adaptin antibody [EPR7572]
See all Alpha1-adaptin primary antibodies -
Description
Rabbit monoclonal [EPR7572] to Alpha1-adaptin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human Alpha1-adaptin aa 50-150. The exact sequence is proprietary.
Database link: O95782 -
Positive control
- HeLa, 293T, HepG2 and A431 cell lysates; Human prostate and liver tissues; Hela cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7572 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Alpha1-adaptin antibody [EPR7572] (ab151720) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 108 kDa
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Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling alpha Adaptin with ab151720 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling alpha Adaptin with ab151720 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow cytometric analysis of permeabilized HeLa cells labeling alpha Adaptin with ab151720 at 1/100 diluton (red), compared to a nonspecific control antibody (green).
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