Anti-PSD95 (phospho T19) antibody (ab16496)
Key features and details
- Rabbit polyclonal to PSD95 (phospho T19)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-PSD95 (phospho T19) antibody
See all PSD95 primary antibodies -
Description
Rabbit polyclonal to PSD95 (phospho T19) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
-
Immunogen
Synthetic peptide corresponding to Mouse PSD95 aa 1-100 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab17167) -
Positive control
- mouse brain
-
General notes
Phosphorylation of PSD95 dynamically regulates the clustering of PSD95/NMDA receptors at synapses, providing a possible mechanism for rapid changes in density and/or number of receptor at synapses.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedPrimary antibody notes
Phosphorylation of PSD95 dynamically regulates the clustering of PSD95/NMDA receptors at synapses, providing a possible mechanism for rapid changes in density and/or number of receptor at synapses.Clonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatHumanApplication Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).Target
-
Function
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
Domain
The PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density. -
Post-translational
modificationsPalmitoylation of isoform 1 is required for targeting to postsynaptic density. -
Cellular localization
Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. - Information by UniProt
-
Database links
- Entrez Gene: 1742 Human
- Entrez Gene: 13385 Mouse
- Entrez Gene: 29495 Rat
- Omim: 602887 Human
- SwissProt: P78352 Human
- SwissProt: Q62108 Mouse
- SwissProt: P31016 Rat
- Unigene: 463928 Human
see all -
Alternative names
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
see all
Images
-
Western blot - Anti-PSD95 (phospho T19) antibody (ab16496)Lanes 1 & 3 : ab16496 at 10 µg
Lane 2 : ab16496
Lane 1 : Mouse Brain
Lane 2 : Mouse Brain with with modified blocking peptide at 1 µg/ml
Lane 3 : Mouse Brain with with non-modified blocking peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesIn WB, we recommend blocking in milk. Blocking with BSA has been seen to give high background with this antibody.
Protocols
Datasheets and documents
References (2)
ab16496 has been referenced in 2 publications.
- Delgado JY An Alternative Pin1 Binding and Isomerization Site in the N-Terminus Domain of PSD-95. Front Mol Neurosci 13:31 (2020). PubMed: 32256312
- Zimmermann HR et al. Genetic removal of eIF2a kinase PERK in mice enables hippocampal L-LTP independent of mTORC1 activity. J Neurochem 146:133-144 (2018). WB ; Mouse . PubMed: 29337352
Images
-
Western blot - Anti-PSD95 (phospho T19) antibody (ab16496)Lanes 1 & 3 : ab16496 at 10 µg
Lane 2 : ab16496
Lane 1 : Mouse Brain
Lane 2 : Mouse Brain with with modified blocking peptide at 1 µg/ml
Lane 3 : Mouse Brain with with non-modified blocking peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesIn WB, we recommend blocking in milk. Blocking with BSA has been seen to give high background with this antibody.
