Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SHANK2 antibody [EPR24142-35] (ab259966)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24142-35] to SHANK2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-SHANK2 antibody [EPR24142-35]
    See all SHANK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR24142-35] to SHANK2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse hippocampus tissue lysate; rat brain and hippocampus tissue lysates. IHC-P: Mouse cerebrum and hippocampus tissue; Rat liver and hippocampus tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24142-35

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SHANK2 antibody [EPR24142-35] (ab259966)
    Western blot - Anti-SHANK2 antibody [EPR24142-35] (ab259966)
    All lanes : Anti-SHANK2 antibody [EPR24142-35] (ab259966) at 1/1000 dilution

    Lane 1 : Mouse hippocampus tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat hippocampus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 159 kDa
    Observed band size: 165 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Shank2 is expressed predominately in brain tissue what has described in the literature. (PMID: 9742101).

    Exposure time: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SHANK2 with ab259966 at 1/5000 (0.0924 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic stainig on mouse cerebrum (PMID:29572432, 28179641). The section was incubated with ab259966 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling SHANK2 with ab259966 at 1/5000 (0.0924 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic stainig on mouse hippocampus (PMID: 27581745, 29572432, 28179641). The section was incubated with ab259966 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling SHANK2 with ab259966 at 1/5000 (0.0924 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on bile duct in rat liver (PMID: 14977424). The section was incubated with ab259966 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966)

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling SHANK2 with ab259966 at 1/5000 (0.0924 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic stainig on rat hippocampus. The section was incubated with ab259966 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-SHANK2 antibody [EPR24142-35] (ab259966)
    Anti-SHANK2 antibody [EPR24142-35] (ab259966)

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