Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
![Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)](https://www.abcam.com/ps/products/2/ab2723/Images/ab2723-352022-ab2723AP3930684IHCPRatretinal2ugDS.jpg "Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)")
Key features and details
- Mouse monoclonal [6G6-1C9] to PSD95 - Synaptic Marker
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG2a
Overview
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Product name
Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker
See all PSD95 primary antibodies -
Description
Mouse monoclonal [6G6-1C9] to PSD95 - Synaptic Marker -
Host species
Mouse -
Specificity
We do not guarantee IHC-P for mouse.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatWB Rat
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Immunogen
Recombinant full length protein corresponding to Rat PSD95.
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Positive control
- IHC-P: Rat retina tissue. WB: Rat and mouse hippocampus and forebrain tissue lysate.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Protocols tab contains a Mouse on Mouse staining protocol with recommendations when using a mouse monoclonal antibody to stain mouse tissues and tips for reducing background.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
6G6-1C9 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
IHC image of ab2723 staining in formalin-fixed, paraffin-embedded rat retinal tissue, performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2723, 2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Secondary-only control image is shown as insert.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)](https://www.abcam.com/ps/products/2/ab2723/Images/ab2723-6251-anti-psd95-antibody-6g6-1c9-western-blot.jpg)
Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)All lanes : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 5 µg/ml
Lane 1 : Hippocampus (Rat) Tissue Lysate
Lane 2 : Forebrain (Rat) Tissue Lysate
Lane 3 : Hippocampus (Mouse) Tissue Lysate
Lane 4 : Forebrain (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 110 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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![Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)](https://www.abcam.com/ps/products/2/ab2723/Images/ab2723-263309-anti-psd95-antibody-6g6-1c9-western-blot.jpg)
Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) Image courtesy of an AbReview submitted by Grant CorbettLanes 1-6 : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 1/1200 dilution
Lanes 7-12 : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 1/1200 dilution (18 hours at 4°C)
Lanes 1-6 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
Lanes 7-12 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Li-Cor IRDye® Donkey anti-mouse 680LT at 1/18000 dilution
Performed under reducing conditions.
Predicted band size: 80 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
