Anti-PSD95 antibody [K28/43] - Synaptic Marker (ab192757)
![Anti-PSD95 antibody [K28/43] - Synaptic Marker (ab192757)](https://www.abcam.com/ps/products/192/ab192757/Images/ab192757-4-anti-psd95-k2843-synaptic-marker-immunocytochemistry-antibody-primary-neurons-div14-rat.jpg "Anti-PSD95 antibody [K28/43] - Synaptic Marker (ab192757)")
Key features and details
- Mouse monoclonal [K28/43] to PSD95 - Synaptic Marker
- Suitable for: ICC, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-PSD95 antibody [K28/43] - Synaptic Marker
See all PSD95 primary antibodies -
Description
Mouse monoclonal [K28/43] to PSD95 - Synaptic Marker -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC RatIHC-P HumanWB Mouse
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Immunogen
Fusion protein corresponding to Human PSD95 (internal sequence). Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
K28/43 -
Isotype
IgG2a -
Research areas
Images
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Immunocytochemistry - Anti-PSD95 antibody [K28/43] - Synaptic Marker (ab192757)
ab192757 staining PSD95 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab192757 at 1µg/ml and ab41455, Rabbit Poly to Human SNAP25 (No Modifications). Cells were then incubated with ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in red) and ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in pseudocolour green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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![Western blot - Anti-PSD95 antibody [K28/43] - Synaptic Marker (ab192757)](https://www.abcam.com/ps/products/192/ab192757/Images/ab192757-253085-anti-psd95-antibody-k28-43-western-blot.jpg)
Western blot - Anti-PSD95 antibody [K28/43] - Synaptic Marker (ab192757)All lanes : Anti-PSD95 antibody [K28/43] - Synaptic Marker (ab192757) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Human Cerebral Cortex Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 80,95 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [K28/43] - Synaptic Marker (ab192757)](https://www.abcam.com/ps/products/192/ab192757/Images/ab192757-252941-anti-psd95-antibody-k28-43-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [K28/43] - Synaptic Marker (ab192757)IHC image of PSD95 [K28/43] staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab192757, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
