Anti-ZNF449 antibody [OTI4C6] (ab236427)
Key features and details
- Mouse monoclonal [OTI4C6] to ZNF449
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-ZNF449 antibody [OTI4C6] -
Description
Mouse monoclonal [OTI4C6] to ZNF449 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla -
Immunogen
Recombinant full length protein corresponding to Human ZNF449 aa 1 to the C-terminus. (NP_689908) produced in E.coli.
Database link: Q6P9G9 -
Positive control
- WB: pCMV6-ENTRY ZNF449 transfected HEK-293T cell lysate. IHC-P: Human kidney carcinoma, lung carcinoma and bladder tissue.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI4C6 -
Isotype
IgG1 -
Research areas
Images
-
All lanes : Anti-ZNF449 antibody [OTI4C6] (ab236427) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY ZNF449 cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 60 kDa
-
Paraffin-embedded human kidney carcinoma tissue stained for ZNF449 using ab236427 at 1/150 dilution in immunohistochemical analysis.
-
Paraffin-embedded human lung carcinoma tissue stained for ZNF449 using ab236427 at 1/150 dilution in immunohistochemical analysis.
-
Paraffin-embedded human bladder tissue stained for ZNF449 using ab236427 at 1/150 dilution in immunohistochemical analysis.