Anti-Cx40/GJA5 antibody (ab113534)
Key features and details
- Rabbit polyclonal to Cx40/GJA5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cx40/GJA5 antibody
See all Cx40/GJA5 primary antibodies -
Description
Rabbit polyclonal to Cx40/GJA5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in both JEG3 and A549 whole cell lysates.
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General notes
Previously labelled as Connexin 40 / GJA5.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications HorseDogChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Target
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Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. -
Involvement in disease
Defects in GJA5 are a cause of familial atrial standstill (FAS) [MIM:108770]. Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm. -
Sequence similarities
Belongs to the connexin family. Alpha-type (group II) subfamily. -
Cellular localization
Cell membrane. Cell junction > gap junction. - Information by UniProt
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Database links
- Entrez Gene: 483155 Dog
- Entrez Gene: 2702 Human
- Omim: 121013 Human
- SwissProt: P33725 Dog
- SwissProt: P36382 Human
- Unigene: 447968 Human
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Alternative names
- Connexin 40 antibody
- Connexin 40 / GJA5 antibody
- Connexin-40 antibody
see all
Images
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Western blot - Anti-Cx40/GJA5 antibody (ab113534)All lanes : Anti-Cx40/GJA5 antibody (ab113534) at 1 µg/ml
Lane 1 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 4 minutes
Datasheets and documents
References (1)
ab113534 has been referenced in 1 publication.
- Bu H et al. Overexpression of PRC1 indicates a poor prognosis in ovarian cancer. Int J Oncol 56:685-696 (2020). PubMed: 31922238
Images
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Western blot - Anti-Cx40/GJA5 antibody (ab113534)All lanes : Anti-Cx40/GJA5 antibody (ab113534) at 1 µg/ml
Lane 1 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 4 minutes
