Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cx40/GJA5 antibody (ab113534)

Key features and details

  • Rabbit polyclonal to Cx40/GJA5
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Cx40/GJA5 antibody
    See all Cx40/GJA5 primary antibodies

  • Description

    Rabbit polyclonal to Cx40/GJA5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in both JEG3 and A549 whole cell lysates.

  • General notes

    Previously labelled as Connexin 40 / GJA5. 

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab113534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Horse
Dog
Chimpanzee
Macaque monkey
Gorilla
Orangutan
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

Target

  • Function

    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

  • Involvement in disease

    Defects in GJA5 are a cause of familial atrial standstill (FAS) [MIM:108770]. Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm.

  • Sequence similarities

    Belongs to the connexin family. Alpha-type (group II) subfamily.

  • Cellular localization

    Cell membrane. Cell junction > gap junction.
  • Information by UniProt

  • Database links

    • Alternative names

      • Connexin 40 antibody
      • Connexin 40 / GJA5 antibody
      • Connexin-40 antibody
      • Cx40 antibody
      • CXA5_HUMAN antibody
      • Gap junction alpha 5 protein antibody
      • Gap junction alpha-5 protein antibody
      • Gap junction protein alpha 5 40kD (connexin 40) antibody
      • Gap junction protein alpha 5 antibody
      • Gja5 antibody
      • MGC11185 antibody
      see all

    Images

    • Western blot - Anti-Cx40/GJA5 antibody (ab113534)
      Western blot - Anti-Cx40/GJA5 antibody (ab113534)
      All lanes : Anti-Cx40/GJA5 antibody (ab113534) at 1 µg/ml

      Lane 1 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
      Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 40 kDa
      Observed band size: 40 kDa


      Exposure time: 4 minutes

    References (1)

    Publishing research using ab113534? Please let us know so that we can cite the reference in this datasheet.

    ab113534 has been referenced in 1 publication.

    • Bu H  et al. Overexpression of PRC1 indicates a poor prognosis in ovarian cancer. Int J Oncol 56:685-696 (2020). PubMed: 31922238

    Images

    • Western blot - Anti-Cx40/GJA5 antibody (ab113534)
      Western blot - Anti-Cx40/GJA5 antibody (ab113534)
      All lanes : Anti-Cx40/GJA5 antibody (ab113534) at 1 µg/ml

      Lane 1 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
      Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 40 kDa
      Observed band size: 40 kDa


      Exposure time: 4 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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