Anti-PSD95 antibody [EP2652Y] (ab76115)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2652Y] to PSD95
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PSD95 antibody [EP2652Y]
See all PSD95 primary antibodies -
Description
Rabbit monoclonal [EP2652Y] to PSD95 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide within Human PSD95 (N terminal). The exact sequence is proprietary.
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Positive control
- Human brain, mouse brain and rat brain tissue lysates.
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General notes
IHC result showed non specific positive staining on most of the human, mouse and rat tissues. We would like to recommend ab238135 as alternative.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2652Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PSD95 antibody [EP2652Y] (ab76115) at 1/2000 dilution
Lane 1 : Rat brain homogenate with nuclear fraction removed at 10 µg
Lane 2 : Rat brain homogenate with nuclear fraction removed at 20 µg
Lane 3 : Rat brain homogenate with nuclear fraction removed at 30 µg
Lane 4 : Rat brain homogenate with nuclear fraction removed at 40 µg
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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All lanes : Anti-PSD95 antibody [EP2652Y] (ab76115) at 1/2000 dilution
Lane 1 : Mouse brain homogenate with nuclear fraction removed at 10 µg
Lane 2 : Mouse brain homogenate with nuclear fraction removed at 20 µg
Lane 3 : Mouse brain homogenate with nuclear fraction removed at 30 µg
Lane 4 : Mouse brain homogenate with nuclear fraction removed at 40 µg
Secondary
All lanes : HRP-conjugated goat anti-rabbit polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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All lanes : Anti-PSD95 antibody [EP2652Y] (ab76115) at 1/2000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 95 kDa
Observed band size: 95 kDa
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