Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13378] to VPS18 - N-terminal
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-VPS18 antibody [EPR13378] - N-terminal
    See all VPS18 primary antibodies

  • Description

    Rabbit monoclonal [EPR13378] to VPS18 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human VPS18 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9P253

  • Positive control

    • HeLa, 293T and K562 cell lysates; 293T cells; K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13378

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
    Western blot - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
    All lanes : Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 110 kDa
    Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
    Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)

    Immunofluorescent analysis of 293T cells labeling VPS18 with ab178416 at 1/100 (green) and DAPI staining (blue).

  • Flow Cytometry - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
    Flow Cytometry - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)

    Flow Cytometrical analysis of permeabilized K562 cells labeling VPS18 with ab178416 at 1/10 (red) or a rabbit IgG (negative) (green).

  • Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
    Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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