Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14519(B)] to CAPON/NOS1AP
Suitable for: ICC/IF, WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-CAPON/NOS1AP antibody [EPR14519(B)]
See all CAPON/NOS1AP primary antibodies
Description

Rabbit monoclonal [EPR14519(B)] to CAPON/NOS1AP
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal brain, Human cerebellum, Mouse brain and Rat brain lysates: MCF-7, HeLa, C6, Raw 264.7, PC-12 and NIH/3T3 cell lysates: SH-SY5Y and HeLa cells.
General notes
This product was previously labelled as CAPON

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14519(B)
Isotype

IgG
Research areas

Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)

All lanes : Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686) at 1/1000 dilution

Lane 1 : Human fetal brain lysates
Lane 2 : Human cerebellum brain lysates
Lane 3 : MCF-7 cell lysates
Lane 4 : HeLa cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)

Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686) at 1/10000 dilution + Human fetal brain lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)

All lanes : Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686) at 1/1000 dilution

Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lane 3 : C6 cell lysates
Lane 4 : Raw 264.7 cell lysates
Lane 5 : PC-12 cell lysates
Lane 6 : NIH/3T3 cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)

Immunofluorescence analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling CAPON/NOS1AP with ab190686 at 1/50 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).
Flow Cytometry - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)

Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling CAPON/NOS1AP with ab190686 at 1/130 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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