Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14519(B)] to CAPON/NOS1AP
  • Suitable for: ICC/IF, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CAPON/NOS1AP antibody [EPR14519(B)]
    See all CAPON/NOS1AP primary antibodies

  • Description

    Rabbit monoclonal [EPR14519(B)] to CAPON/NOS1AP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain, Human cerebellum, Mouse brain and Rat brain lysates: MCF-7, HeLa, C6, Raw 264.7, PC-12 and NIH/3T3 cell lysates: SH-SY5Y and HeLa cells.

  • General notes

     

     

     This product was previously labelled as CAPON

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14519(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)
    Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)
    All lanes : Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686) at 1/1000 dilution

    Lane 1 : Human fetal brain lysates
    Lane 2 : Human cerebellum brain lysates
    Lane 3 : MCF-7 cell lysates
    Lane 4 : HeLa cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa

  • Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)
    Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)
    Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686) at 1/10000 dilution + Human fetal brain lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa

  • Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)
    Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)
    All lanes : Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686) at 1/1000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Rat brain lysates
    Lane 3 : C6 cell lysates
    Lane 4 : Raw 264.7 cell lysates
    Lane 5 : PC-12 cell lysates
    Lane 6 : NIH/3T3 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)
    Immunocytochemistry/ Immunofluorescence - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)

    Immunofluorescence analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling CAPON/NOS1AP with ab190686 at 1/50 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).

  • Flow Cytometry - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)
    Flow Cytometry - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)

    Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling CAPON/NOS1AP with ab190686 at 1/130 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)
    Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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