Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)
![Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)](https://www.abcam.com/ps/products/180/ab180172/Images/ab180172-261391-ab180172KOWB.jpg "Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13455(B)] to VPS26 - N-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-VPS26 antibody [EPR13455(B)] - N-terminal
See all VPS26 primary antibodies -
Description
Rabbit monoclonal [EPR13455(B)] to VPS26 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human VPS26 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: O75436 -
Positive control
- WB: HepG2, HeLa and 293T cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13455(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: VPS26 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab180172 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.ab180172 was shown to specifically react with VPS26 when VPS26 knockout samples were used. Wild-type and VPS26 knockout samples were subjected to SDS-PAGE. ab180172 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)](https://www.abcam.com/ps/products/180/ab180172/Images/ab180172-208768-ab180172wb.jpg)
Western blot - Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)All lanes : Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa
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![Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)](https://www.abcam.com/ps/products/180/ab180172/Images/ab180172-3-benefits-of-recombinant-antibodies.png)
Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)
