Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)

Key features and details

  • Mouse monoclonal [CL2287] to VPS26 - C-terminal
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-VPS26 antibody [CL2287] - C-terminal
    See all VPS26 primary antibodies

  • Description

    Mouse monoclonal [CL2287] to VPS26 - C-terminal

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human VPS26 aa 300 to the C-terminus (C terminal).
    Database link: O75436

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • U251 MG cell line lysate; Human fallopian tube, small intestine, testis, and kidney tissues; HeLa , A431 , MCF7, U2OS and U251 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
    Western blot - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
    All lanes : Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)

    Lane 1 : U-251 cells, transfected with control siRNA
    Lane 2 : U-251 cells, transfected with target specific siRNA probe #1
    Lane 3 : U-251 cells, transfected with target specific siRNA probe #2

    Predicted band size: 38 kDa



    Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.

  • Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
    Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)

    Immunofluorescent analysis of U251 cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling VPS26 with ab211530 at 1/200 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
    Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)

    Immunofluorescent analysis of U2OS cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
    Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)

    Immunofluorescent analysis of MCF7 cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
    Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)

    Immunofluorescent analysis of A431 cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
    Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)

    Immunofluorescent analysis of HeLa cells (PFA-fixed/Triton X-100 permeabilized) labeling VPS26 with ab211530 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling VPS26 with ab211530 at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)

    Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling VPS26 with ab211530 at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)

    Immunohistochemical analysis of paraffin-embedded Human fallopian tube tissue labeling VPS26 with ab211530 at 1/200 dilution.

  • Western blot - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
    Western blot - Anti-VPS26 antibody [CL2287] - C-terminal (ab211530)
    Anti-VPS26 antibody [CL2287] - C-terminal (ab211530) at 1/200 dilution + U251 MG cell line lysate

    Predicted band size: 38 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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