Anti-ARFGEF2/BIG2 antibody (ab236951)
Key features and details
- Rabbit polyclonal to ARFGEF2/BIG2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-ARFGEF2/BIG2 antibody
See all ARFGEF2/BIG2 primary antibodies -
Description
Rabbit polyclonal to ARFGEF2/BIG2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human ARFGEF2/BIG2 aa 200-350.
Database link: Q9Y6D5 -
Positive control
- IHC-P: Human breast cancer and pancreatic cancer tissue. ICC/IF: A549 cells. WB: Rat heart, Mouse brain, and Mouse liver tissue lysate
-
General notes
This product was previously labelled as ARFGEF2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-ARFGEF2/BIG2 antibody (ab236951)All lanes : Anti-ARFGEF2/BIG2 antibody (ab236951) at 3.2 µg/ml
Lane 1 : Rat heart tissue
Lane 2 : Mouse brain tissue
Lane 3 : Mouse liver tissue
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 202 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-ARFGEF2/BIG2 antibody (ab236951)A549 (Human lung carcinoma cell line) cells stained for ARFGEF2/BIG2 using ab236951 at a dilution of 1/133 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). COunterstained with DAPI (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGEF2/BIG2 antibody (ab236951)Paraffin-embedded human breast cancer tissue stained for ARFGEF2/BIG2 with ab236951 at a 1/100 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGEF2/BIG2 antibody (ab236951)Paraffin-embedded human pancreatic cancer tissue stained for ARFGEF2/BIG with ab236951 at a 1/100 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
