Anti-Hepatitis B Surface Antigen antibody [5C3] (ab277481)
Key features and details
- Mouse monoclonal [5C3] to Hepatitis B Surface Antigen
- Suitable for: IHC-P
- Reacts with: Hepatitis B virus
- Isotype: IgG2a
Overview
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Product name
Anti-Hepatitis B Surface Antigen antibody [5C3] -
Description
Mouse monoclonal [5C3] to Hepatitis B Surface Antigen -
Host species
Mouse -
Specificity
This antibody stains the cytoplasm of antigen-positive liver cells in patients with Type B viral hepatitis. This antibody reacts with the ‘a’ determinant present on the HBsAg subtypes ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4 and adr. It does not react with normal tissues.
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Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Hepatitis B virus -
Immunogen
Full length protein corresponding to Hepatitis B virus Hepatitis B Surface Antigen. HBsAg (ad/ay) protein.
Database link: Q773S4 -
Positive control
- IHC-P: Hepatitis B virus-infected human liver tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 98.9% Tris buffer, 1% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
5C3 -
Isotype
IgG2a -
Light chain type
kappa
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Surface Antigen antibody [5C3] (ab277481)
Immunohistochemical analysis (Formalin-fixed, paraffin-embedded) of HBV-infected human liver stained for Hepatitis B Surface Antigen using ab277481 at 1/25 dilution. Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Surface Antigen antibody [5C3] (ab277481)
Formalin-fixed, paraffin-embedded HBV-infected human liver stained for Hepatitis B Surface Antigen using ab277481 at 1/25 dilution in immunohistochemical analysis.