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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-eIF4E antibody (ab245299)

Anti-eIF4E antibody (ab245299)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to eIF4E
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-eIF4E antibody
    See all eIF4E primary antibodies
  • Description

    Rabbit polyclonal to eIF4E
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human eIF4E aa 167-217. The exact sequence is proprietary. NP_001959.1
    Database link: P06730

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245299 was affinity purified using an epitope specific to eIF4E immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

Images

  • Western blot - Anti-eIF4E antibody (ab245299)
    Western blot - Anti-eIF4E antibody (ab245299)
    All lanes : Anti-eIF4E antibody (ab245299) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 25 kDa


    Exposure time: 30 seconds


    Lysates prepared in NETN buffer.

  • Immunoprecipitation - Anti-eIF4E antibody (ab245299)
    Immunoprecipitation - Anti-eIF4E antibody (ab245299)

    eIF4E was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245299 at 3 µg per reaction. Western blot was performed from the immunoprecipitate using ab245299 at 1 µg/ml.

    Lane 1: ab245299 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 75 seconds.

    Lysates prepared in NETN buffer.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-eIF4E antibody (ab245299)

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    Anti-eIF4E antibody [Y448] (ab33766)

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    Anti-eIF4E (phospho S209) antibody [EP2151Y] (ab76256)

    Applications: Dot, ICC/IF, IHC-P, IP, WB

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    Anti-eIF4E antibody [Y448] - BSA and Azide free (ab240923)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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